Biolegend, 344206, APC anti-human CD143 (Angiotensin-converting enzyme) Antibody, 100tests

CD143 (ACE, kininase II, peptidyl dipeptidase 1, peptidase P, carboxycathepsin) is a 171 kD, type I, single chain transmembranal metallopeptidase, whose cofactor is zinc. Its main targets are angiotensin I and bradykinin, acting as a blood pressure regulator. CD143 is expressed in endothelial cells; varying amounts of CD143 have been reported in different epithelial cells. The activation of macrophages and histiocytes induces the expression of this molecule.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Pope C, et al. 2016. Circ Res . 119: 1204 - 1214. PubMed
RRID: AB_11149487 (BioLegend Cat. No. 344206)
Structure: Single chain, type I transmembranal zinc metallopeptidase, 170 kD.
Distribution: Endothelial cells, some types of epithelial cells, activated macrophages and histiocytes.
Function: Regulates blood pressure by promoting vasoconstriction by the conversion of angiotensin I to angiotensin II and by the degradation of bradykinin, a vasodilator.
Ligand/Receptor: Angiotensin I, bradykinin.
Bioactivity: Blood pressure regulator.
Cell Type: Endothelial cells, Epithelial cells, Macrophages
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Nakamura T, et al. 2009. Int Heart J. 50:501. 2. Jayasooriya AP, et al. 2008. P. Natl. Acad. Sci. USA 105:6531. 3. Jokubaitis VJ, et al. 2008. Blood 111:4055. 4. Arndt PG, et al. 2006. J. Immunol. 177:7233. 5. Balyasnikova IV, et al. 2002. Biochem J. 362:585.
Gene ID: 1636
UniProt: View information about CD143 on UniProt.org
Clone: 5-369
Regulatory Status: RUO
Other Names: ACE, kininase II, dipeptidyl dipeptidase 1, peptidase P, carboxycathepsin
Isotype: Mouse IgG1, κ