Biolegend, 345203, PE anti-human integrin β5 Antibody, 25tests

Integrins are a family of transmembrane proteins. A total 17 integrin α chains and 8 β chains have been identified. The association of integrin α and β chains forms 25 integrin complexes which serve as extracellular matrix receptors and play important roles in cell adhesion and development. Integrin β5 is a 95 kD glycoprotein heterodimer with the αV subunit and is found on many types of tissue cells, such as epithelial cells, endothelial cells, keratinocytes, and osteoblastic cells. The αV/β5 integrin complex binds to vitronectin.
25tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Perna F, et al. 2017. Cancer Cell. 32:506. PubMed Zuidema A, et al. 2018. J Cell Sci. 131. PubMed Kawamoto E, et al. 2021. Biomedicines. 9:. PubMed
RRID: AB_2129167 (BioLegend Cat. No. 345203)
Structure: Integrin family, Ig superfamily, associates with αV subunit
Distribution: Epithelial cells, endothelial cells, keratinocytes, osteoblastic cells
Function: Cell adhesion
Ligand/Receptor: vitronectin
Cell Type: Endothelial cells, Epithelial cells, Osteoclasts
Biology Area: Immunology
Molecular Family: Adhesion Molecules
Antigen References: 1. Kim JP. et al. 1994. J. Bio. Chem. 269:26926 2. Cheng SL. et al. 2000. J. Cell Biochem. 77:265 3. Aplin JD. et al. 1996. Mol. Hum. Reprod. 2:527
Gene ID: 3693
UniProt: View information about Integrin beta 5 on UniProt.org
Clone: AST-3T
Regulatory Status: RUO
Other Names: integrin b5, integrin beta 5, ITGB5
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.