Biolegend, 347101, Purified anti-human Siglec-8 Antibody, 25μg

Siglec-8 is a lectin specific for 6'-sulfo-sLe x and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Siglec-8 fused to human IgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Floyd H, et al. 2000. J. Biol. Chem. 275:861. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations: Kano G, et al. 2013. J Allergy Clin Immunol. 132:437. PubMed Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed Meng X, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed Arakawa S, et al. 2018. Allergol Int. 67S:S41. PubMed Wen T, et al. 2014. J Immunol. 192:5481. PubMed Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
RRID: AB_2239311 (BioLegend Cat. No. 347102)
Structure: A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain
Distribution: Eosinophils, basophils, mast cells
Function: Cell adhesion, signal transduction
Interaction: Phosphatases containing a SH2 domain
Bioactivity: Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
Cell Type: Basophils, Eosinophils, Mast cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, Siglec Molecules
Antigen References: 1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317. 2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608. 3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.
Gene ID: 27181
UniProt: View information about Siglec-8 on UniProt.org
Clone: 7C9
Regulatory Status: RUO
Other Names: Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
Isotype: Mouse IgG1, κ