Biolegend, 347103, PE anti-human Siglec-8 Antibody, 25tests

Siglec-8 is a lectin specific for 6'-sulfo-sLe x and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Siglec-8 fused to human IgG Fc
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Floyd H, et al. 2000. J. Biol. Chem. 275:861. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
Product Citations: Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed Youngblood BA, et al. 2020. Cells. 10:. PubMed Wong T, Jelinek D 2013. J Immunol Methods. 387:130. PubMed Gadkari M, et al. 2018. Sci Data. 5:180275. PubMed Lin J, et al. 2017. Sci Rep. 7:41722. PubMed Duan S, et al. 2021. J Immunol. 206:2290. PubMed Hernandez JD, et al. 2020. Sci Rep. 10:13210. PubMed Serra EG, et al. 2020. Nat Commun. 11:995. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Wright A, et al. 2016. J Immunol. 196: 4877 - 4884. PubMed Fan Z, et al. 2020. STAR Protoc. 1:100012. PubMed Legrand F, et al. 2019. J Allergy Clin Immunol. 143:2227. PubMed Motwani MP, et al. 2018. JCI Insight. 3:e94463. PubMed Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed Jenner W, et al. 2014. PLoS One. 9:89375. PubMed Lippeveld M, et al. 2020. Cytometry A. 97:308. PubMed
RRID: AB_2189426 (BioLegend Cat. No. 347103) AB_2270438 (BioLegend Cat. No. 347104)
Structure: A lectin of 54 kD, belonging to the Ig-superfamily; has one V-set Ig-like domain and two C2-set domains; has a single transmembrane domain, and two immunoreceptor tyrosine-based inhibitor motifs (ITIM) in the cytoplasmic domain
Distribution: Eosinophils, basophils, mast cells
Function: Cell adhesion, signal transduction
Interaction: Phosphatases containing a SH2 domain
Bioactivity: Inhibition of IgE receptor-triggered histamine and prostaglandin D2 release, apoptosis
Cell Type: Basophils, Eosinophils, Mast cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Adhesion Molecules, Siglec Molecules
Antigen References: 1. Bochner BS, et al. 2009. Clin. Exp. Allergy. 39:317. 2. Hudson SA, et al. 2009. J. Pharmacol. Exp. Ther. 330:608. 3. Nutku E, et al. 2005. Biochem. Biophys. Res. Commun. 336:918.
Gene ID: 27181
UniProt: View information about Siglec-8 on UniProt.org
Clone: 7C9
Regulatory Status: RUO
Other Names: Sialic acid-binding Ig-like lectin 8 (Siglec-8), Siglec8L, Sialoadhesin family member 2 (SAF2)
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.