Biolegend, 348805, Pacific Blue™ anti-human Lineage Cocktail (CD3, CD14, CD16, CD19, CD20, CD56), 50tests

This anti-Human Lineage Cocktail is optimized for the detection of human peripheral blood T cells, B cells, NK cells, monocytes, and neutrophils. In combination with other markers, it can be used for studies of dendritic cells and/or basophils. This cocktail is composed of CD3, CD14, CD16, CD19, CD20, and CD56. CD3 is the antigen mainly found on T cells; CD14 is expressed on monocytes/macrophages and at low levels on neutrophils and eosinophils; CD16 is expressed on NK cells, activated monocytes/macrophages, and neutrophils; CD19 and CD20 are on B cells; CD56 is located on NK cells.
50tests
Verified Reactivity: Human
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 20 µl per million cells or 20 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules. New Jersey. (FC)
Product Citations: Wang X, et al. 2021. Cell Discovery. 7(1):60. PubMed Hu EY, et al. 2020. JCI Insight. 5:00. PubMed Schielke L, et al. 2022. Front Immunol. 13:916701. PubMed Moravcikova E, et al. 2018. Cytometry A. 93:894. PubMed Saygin C, et al. 2021. Leukemia. 35:3406. PubMed Verma M, et al. 2021. J Exp Med. 218:. PubMed
RRID: AB_2889063 (BioLegend Cat. No. 348805)
Distribution: T cells, B cells, NK cells, monocytes/macrophages, neutrophils
Cell Type: B cells, Dendritic cells, Macrophages, Mesenchymal Stem Cells, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Gene ID: 91522149309314684929
UniProt: View information about Lineage on UniProt.org
Clone: UCHT1; HCD14; 3G8; HIB19; 2H7; HCD56;
Regulatory Status: RUO
Other Names: Lin 1
Isotype: Mouse IgG1, κ/Mouse IgG2b, κ