Biolegend, 349103, FITC anti-human CD235a (Glycophorin A) Antibody, 25μg

CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes.  It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
Product Citations: Qin K, et al. 2022. Nat Genet. 54:874. PubMed Perik-Zavodskii R, et al. 2022. Genes (Basel). 13: . PubMed Abe I, et al. 2022. Dev Cell. 57:2623. PubMed Wheeler MM, et al. 2022. Nat Commun. 13:7592. PubMed Segal JM, et al. 2019. Nat Commun. 10:3350. PubMed Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed Xu Y, et al. 2021. Stem Cell Res Ther. 12:112. PubMed Wu Y, et al. 2019. Nat Med. 25:776. PubMed Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed Lan X, et al. 2020. Molecular Cell. 81(2):239-254.e8. PubMed Heatlie J, et al. 2020. Biopreserv Biobank. 1.070833333. PubMed Ruiz JP, et al. 2019. Stem Cell Res. 41:101600. PubMed Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed Zeng J, et al. 2020. Nat Med. 26:535. PubMed Martin RM et al. 2019. Cell Stem Cell. 24(5):821-828 . PubMed Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed Lundin V, et al. 2020. Dev Cell. 446:52. PubMed Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed Grajcarek J, et al. 2019. Nat Commun. 3.788888889. PubMed Weiss R, et al. 2021. Cells. 10:. PubMed Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed Perik-Zavodskii R, et al. 2022. Genes (Basel). 13:. PubMed
RRID: AB_10612923 (BioLegend Cat. No. 349103) AB_10613463 (BioLegend Cat. No. 349104)
Structure: Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer
Distribution: Erythrocytes and erythroid precursors
Function: Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
Ligand/Receptor: Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
Cell Type: Erythrocytes
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Reid ME. 2009. Immunohematology 25:95. 2. Palacajornsuk P. 2006. Immunohematology 22:171. 3. Pasvol G. 2003. Trends Parasitol. 19:430. 4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80.
Gene ID: 2993
UniProt: View information about CD235a on UniProt.org
Clone: HI264
Regulatory Status: RUO
Workshop: VII 70312
Other Names: PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, GYPA
Isotype: Mouse IgG2a, κ