Biolegend, 349406, PE anti-human TCR Vβ23 Antibody, 100tests

Vβ23 T cell receptor (TCR Vβ23) is a variant of the TCR β chain which is heterodimerized with TCR α chain and expressed on a subset of αβ + T cells. TCR α/β recognizes peptides presented by the MHC molecules and initiates T cell mediated immune responses. Vβ23 + T cells are stimulated by the staphylococcal enterotoxin A (SEA) and by the pyrogenic toxin superantigens (PTSAgs) SpeK, SpeL, and SpeM from streptococcus.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: T-cell leukemia line HUT78
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: stimulation of HUT78 T cells to produce IL-21.
Application References(PubMed link indicates BioLegend citation): Romagne F, et al. 1995. J. Immunol. Methods 186:313. (Stim) Rebai N, et al. 1994. P. Natl. Acad. Sci. USA 91:1529. (FC) Pantaleo G, et al. 1997. P. Natl. Acad. Sci. USA 94:9848. (FC)
RRID: AB_10918433 (BioLegend Cat. No. 349406)
Structure: Immunoglobulin superfamily, associate with TCR Vα chain and CD3 complex
Distribution: subset of TCR αβ+ T cells
Function: Recognition of peptides presented by MHC molecules, responsible for T cell mediated immune response
Ligand/Receptor: MHC-peptide complex
Cell Type: T cells
Biology Area: Adaptive Immunity, Immunology
Molecular Family: TCRs
Antigen References: 1. Guilherme L, et al. 2007. Curr. Protein Pept. Sc. 8:39. 2. Adelman MK. 2007. Crit. Rev. Immunol. 27:221. 3. Beres SB, et al. 2002. P. Natl. Acad. Sci. USA 99:10078. 4. Smoot LM, et al. 2002. Infect. Immunol. 70:7095. 5. Lavoie PM, et al. 2005. Int. Immunol. 17:931.
Gene ID: 6957
UniProt: View information about TCR Vbeta23 on UniProt.org
Clone: αHUT7
Regulatory Status: RUO
Other Names: T cell receptor Vbeta 23
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.