Biolegend, 349502, Purified anti-human CD81 (TAPA-1) Antibody, 100μg

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human OCI-LY8 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested WB, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3.
Application References(PubMed link indicates BioLegend citation): Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP) Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB) Mochida K, et al. 2008. J. Virol. 13:6711. Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed
Product Citations: Shi C, et al. 2019. FASEB J. 33:1617. PubMed Ciftci Dede E, et al. 2023. PLoS One. 18:e0282238. PubMed Mascarau R, et al. 2023. J Cell Biol. 222:. PubMed Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Vrancx C, et al. 2021. Mol Neurobiol. 58:6647. PubMed Rodriguez L, et al. 2020. Cell Reports Medicine. 1(5):100078. PubMed Chetty VK, et al. 2022. Cancers (Basel). 14:. PubMed Desjardins P, et al. 2022. Int J Mol Sci. 23:. PubMed Porter RL, et al. 2022. J Clin Invest. :. PubMed van der Heide V, et al. 2022. Cell Rep. :110508. PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Ter-Ovanesyan D, et al. 2021. Elife. 10:. PubMed Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed Araki Y, et al. 2021. Front Oncol. 11:667109. PubMed Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed Zheng W, et al. 2022. Nat Biomed Eng. 6:979. PubMed Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed Bachurski D, et al. 2019. J Extracell Vesicles. 8:1596016. PubMed Lobastova L, et al. 2021. Frontiers in Cell and Developmental Biology. 9:698503. PubMed Shi C, et al. 2020. Biomedicines. 8:00. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed You Y, et al. 2022. J Extracell Vesicles. 11:e12183. PubMed Menck K, et al. 2017. J Extracell Vesicles. 10.1080/20013078.2017.1340745. PubMed Dörsam B, et al. 2018. Front Immunol. 7:e34961. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID: AB_10642023 (BioLegend Cat. No. 349501) AB_10643417 (BioLegend Cat. No. 349502)
Structure: 26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8.
Distribution: T cells, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes.
Function: Regulates cell activation and growth and cell aggregation.
Cell Type: B cells, Endothelial cells, Epithelial cells, Monocytes, Neural Stem Cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127. Wright M, et al. 1994. Immunol. Today 15:588.
Gene ID: 975
UniProt: View information about CD81 on UniProt.org
Clone: 5A6
Regulatory Status: RUO
Other Names: S5.7, CVID6, TSPAN28
Isotype: Mouse IgG1, κ