Biolegend, 349508, PerCP/Cyanine5.5 anti-human CD81 (TAPA-1) Antibody, 100tests

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human OCI-LY8 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3.
Application References(PubMed link indicates BioLegend citation): Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP) Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB) Mochida K, et al. 2008. J. Virol. 13:6711. Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed
Product Citations: Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Elgamal S, et al. 2021. JCI Insight. 6:. PubMed
RRID: AB_2564018 (BioLegend Cat. No. 349507) AB_2564019 (BioLegend Cat. No. 349508)
Structure: 26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8.
Distribution: T cells, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes.
Function: Regulates cell activation and growth and cell aggregation.
Cell Type: B cells, Endothelial cells, Epithelial cells, Monocytes, Neural Stem Cells, NK cells, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127. Wright M, et al. 1994. Immunol. Today 15:588.
Gene ID: 975
UniProt: View information about CD81 on UniProt.org
Clone: 5A6
Regulatory Status: RUO
Other Names: S5.7, CVID6, TSPAN28
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.