Biolegend, 350307, APC anti-human CD1d Antibody, 25tests

CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to i NKT cells analogous to MHC molecule presentation of peptides to T cells.
25tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD1d-Fc fusion
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.
Application References(PubMed link indicates BioLegend citation): Exley M, et al. 2000. Immunology 100:37. (IHC, WB) Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB) Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
Product Citations: Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed Yamaguchi S, et al. 2021. Eur J Immunol. 51:2306. PubMed Deng C, et al. 2016. Diabetes Care. 39: 434 - 440. PubMed Deng C, et al. 2017. J Diabetes Res. 10.1155/2017/5052812. PubMed Vlugt L, et al. 2014. J Infect Dis. 210:1207. PubMed Muir LA, et al. 2022. JCI Insight. 7:. PubMed Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed Rotolo A et al. 2018. Cancer cell. 34(4):596-610 . PubMed
RRID: AB_10642029 (BioLegend Cat. No. 350307) AB_10642829 (BioLegend Cat. No. 350308)
Structure: MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution: Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells
Function: Present lipid antigens to iNKT cells
Ligand/Receptor: When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens, TCRs
Antigen References: 1. Koch M, et al. 2005. Nat. Immunol. 6:819. 2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010. 3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889. 4. Teige A, et al. 2010. J. Immunol. 185:345.
Gene ID: 912
UniProt: View information about CD1d on UniProt.org
Clone: 51.1
Regulatory Status: RUO
Other Names: R3G1
Isotype: Mouse IgG2b, κ