Biolegend, 350321, Ultra-LEAF™ Purified anti-human CD1d Antibody, 100μg

CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to i NKT cells analogous to MHC molecule presentation of peptides to T cells.
100μg
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD1d-Fc fusion
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality testedIHC, IP, Stim, WB, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.
Application References(PubMed link indicates BioLegend citation): Exley M, et al. 2000. Immunology 100:37. (IHC, WB) Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB) Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
RRID: AB_2814280 (BioLegend Cat. No. 350321) AB_2814281 (BioLegend Cat. No. 350322)
Structure: MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution: Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells
Function: Present lipid antigens to iNKT cells
Ligand/Receptor: When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type: B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens, TCRs
Antigen References: 1. Koch M, et al. 2005. Nat. Immunol. 6:819. 2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010. 3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889. 4. Teige A, et al. 2010. J. Immunol. 185:345.
Gene ID: 912
UniProt: View information about CD1d on UniProt.org
Clone: 51.1
Regulatory Status: RUO
Other Names: R3G1
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.