Biolegend, 351321, PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα) Antibody, 25tests

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD127
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported (for the relevant formats) application: proteogenomics1.
Additional Product Notes: View more applications data for this product in our Scientific Poster Library.
Application References(PubMed link indicates BioLegend citation): Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations: Yan YN, et al. 2023. Mediators Inflamm. 2023:8215567. PubMed Côrte-Real BF, et al. 2022. Front Immunol. 13:1005965. PubMed Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed Roukens AHE, et al. 2022. Nat Immunol. 23:23. PubMed Wu J, et al. 2021. Front Immunol. 12:601611. PubMed Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed Goodwin M, et al. 2020. Sci Adv. 6:eaaz0571. PubMed Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed Shen C, et al. 2021. Front Immunol. 12:680055. PubMed OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed Van Zeebroeck L, et al. 2021. Front Immunol. 12:655122. PubMed Santoni de Sio FR, et al. 2018. J Allergy Clin Immunol. 142:1909. PubMed Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed
RRID: AB_10900253 (BioLegend Cat. No. 351321) AB_10897104 (BioLegend Cat. No. 351322)
Structure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125. 2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503. 3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610. 4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403. 5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707. 6. Liu W, et al. 2006. J. Exp. Med. 203:1701.
Gene ID: 3575
UniProt: View information about CD127 on UniProt.org
Clone: A019D5
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.