Biolegend, 352805, APC anti-human CD126 (IL-6Rα) Antibody, 25tests

CD126 is an 80 kD IL-6 receptor α chain also known as IL-6R. It is a member of the immunoglobulin superfamily that is expressed on plasma cells, T cells, activated B cells, monocytes, granulocytes, hepatocytes, epithelial cells, and fibroblasts. Functional IL-6 receptors are formed by the non-covalent association of CD126 and the IL-6 receptor β chain (CD130 or gp130). CD126 binds IL-6 with low affinity but does not signal. The β chain (gp130, CD130) does not bind IL-6 by itself but associates with the α-chain/IL-6 complex to initiate signal transduction. IL-6 binding to the receptor complex results in the stimulation of B and T cells, and hematopoietic precursor proliferation and differentiation. A soluble form of CD126 has been found in human serum.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myeloma cell line U266
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking of IL-6 binding to IL-6R.
Application References(PubMed link indicates BioLegend citation): Huang YW and Vitetta ES. 1993. Hybridoma 12:621.
Product Citations: Ferreira LB, et al. 2023. J Ophthalmic Inflamm Infect. 13:21. PubMed Krow-Lucal E, et al. 2014. Blood. 123:1897. PubMed Domenger A, et al. 2022. EMBO Mol Med. 14:e14740. PubMed Eichten A, et al. 2016. Cancer Res . 76: 2327-2339. PubMed Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed Wolf J, et al. 2016. J Biol Chem. 291: 16186 - 16196. PubMed
RRID: AB_11203543 (BioLegend Cat. No. 352805) AB_11204258 (BioLegend Cat. No. 352806)
Structure: Ig superfamily, associates with IL-6Rβ chain (CD130, gp130), 80 kD
Distribution: Plasma cells, T cells, monocytes, hepatocytes, activated B cells, granulocytes, epithelial cells, and fibroblasts
Function: Stimulates T cells, B cells, and hematopoietic precursor proliferation and differentiation
Interaction: CD130, c-Src, STAT3, WWP1, WWP2
Ligand/Receptor: IL-6
Cell Type: B cells, Epithelial cells, Fibroblasts, Granulocytes, Monocytes, Plasma cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience, Signal Transduction
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taga T, et al. 1997. Annu. Rev. Immunol. 15:797. 2. Fitzgerald K, et al. 2001. The Cytokine FactsBook. Academic Press London. 3. Boulanger MJ, et al. 2003. Science 300:2101. 4. Gaillard JP, et al. 1993. Eur. J. Immunol. 23:820.
Gene ID: 3570
UniProt: View information about CD126 on UniProt.org
Clone: UV4
Regulatory Status: RUO
Other Names: IL-6R1, gp80, IL-6 receptor alpha, IL-6R
Isotype: Mouse IgG1, κ