Biolegend, 353203, PE anti-human CD197 (CCR7) Antibody, 25tests

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Pfister F, et al. 2023. Methods Mol Biol. 2644:331. PubMed Zhang C, et al. 2023. Front Immunol. 14:1182409. PubMed Aschmoneit N, et al. 2021. J Immunother Cancer. 9:. PubMed Cox MJ, et al. 2022. Leukemia. 36:1635. PubMed Chen Y, et al. 2022. Sci Immunol. 7:eabp8328. PubMed Ogura H, et al. 2022. Nat Commun. 13:7063. PubMed Cheng H, et al. 2023. Nat Metab. 5:314. PubMed Giles JR, et al. 2022. Immunity. 55:557. PubMed Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed Zeng W, et al. 2017. Front Immunol. 0.806944444. PubMed Zhou R, et al. 2020. Immunity. S1074-7613(20)30333-2.. PubMed Li M, et al. 2022. Sci Rep. 12:378. PubMed Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed Cappellano G, et al. 2018. PLoS One. 13:e0192108. PubMed Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed Xiong X, et al. 2021. Liver Int. 41:1033. PubMed Kang L, et al. 2020. Exp Hematol Oncol. 9:11. PubMed Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed Kuo HH, et al. 2018. Immunity. 48:1183. PubMed Zhang X, et al. 2021. Nat Commun. 12:5291. PubMed Gullà A, et al. 2021. Cancer Discov. . PubMed Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed Balan I, et al. 2022. Front Immunol. 13:940095. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Ahmad S,et al. 2017. Prog Neuropsychopharmacol Biol Psychiatry. . 10.1016/j.pnpbp.2017.10.001. PubMed Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed Borowicz S, et al. 2021. PLOS ONE. 16(6):e0252197. PubMed Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed Christiansen M, et al. 2020. Front Immunol. 10:3022. PubMed Vadrevu KM, et al. 2022. Sci Rep. 12:12038. PubMed Tavira B, et al. 2018. J Diabetes Res. 2018:9391845. PubMed Audigé A, et al. 2017. BMC Immunology. 10.1186/s12865-017-0209-9. PubMed Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed Ramos MIP, et al. 2021. Elife. 10:. PubMed Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed Chen Y, et al. 2020. Cell. 1496:183. PubMed Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed Gullà A, et al. 2021. Cancer Discov. 2:468. PubMed Chen R, et al. 2021. Front Oncol. 11:743050. PubMed Gamradt S, et al. 2021. iScience. 24:103312. PubMed
RRID: AB_10916391 (BioLegend Cat. No. 353203) AB_10913813 (BioLegend Cat. No. 353204)
Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236
UniProt: View information about CD197 on UniProt.org
Clone: G043H7
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.