Biolegend, 353214, APC anti-human CD197 (CCR7) Antibody, 100tests

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Prockop SE, et al. 2023. J Clin Invest. 133:. PubMed Li M, et al. 2021. J Clin Invest. 131:. PubMed Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed Andonian BJ, et al. 2022. Sci Rep. 12:7450. PubMed Shen Y, et al. 2021. Cell Stress Chaperones. 26:937. PubMed Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed Blengio F, et al. 2013. Immunobiology. 218:76. PubMed Fajgenbaum DC, et al. 2019. J Clin Invest. 130:4451. PubMed Liu C, et al. 2021. Cell Res. 31:1106. PubMed Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed Jo T, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed Miao L, et al. 2021. Clin Transl Med. 11:e395. PubMed Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed Dai X, et al. 2021. Bioact Mater. 1.659027778. PubMed Cho JH, et al. 2021. Nat Commun. 12:792. PubMed Soriano-Sarabia N, et al. 2014. J Virol. 88:14070. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Bradley T et al. 2018. Cell reports. 25(1):107-117 . PubMed Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed Ronaghan NJ, et al. 2022. PLoS One. 17:e0276013. PubMed Mysore V, et al. 2021. Med (N Y). 2:1050. PubMed
RRID: AB_10915474 (BioLegend Cat. No. 353213) AB_10917387 (BioLegend Cat. No. 353214)
Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236
UniProt: View information about CD197 on UniProt.org
Clone: G043H7
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.