Biolegend, 353217, Alexa Fluor® 647 anti-human CD197 (CCR7) Antibody, 25tests

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CCR7-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Ando M, et al. 2021. Cancer Res Commun. 1:41. PubMed Deering RP, et al. 2023. Sci Rep. 13:8452. PubMed Kim MY, et al. 2021. JCI Insight. 6:. PubMed Ogishi M, et al. 2023. J Exp Med. 220: . PubMed Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed Causi E, et al. 2015. PLoS One. 10: 0136717. PubMed Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed Ecker C, et al. 2018. Cell Rep. 23:741. PubMed Zhang W, et al. 2021. Sci Adv. 7: . PubMed Medvec AR, et al. 2018. Mol Ther Methods Clin Dev. 8:65. PubMed Thommen D, et al. 2015. Cancer Immunol Res. 3: 1344 - 1355. PubMed Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed Vikkurthi R, et al. 2022. Nat Microbiol. 7:974. PubMed Rakshit S, et al. 2020. Cell Rep. 33:108451. PubMed Hunter S, et al. 2018. J Hepatol. 69:654. PubMed Qin XH, et al. 2019. Langmuir. 35:1882. PubMed Kim MY, et al. 2022. Nat Commun. 13:3296. PubMed Tanner MR et al. 2017. Clinical immunology (Orlando, Fla. 180:45-57 . PubMed Shan L, et al. 2017. Immunity. 47:766. PubMed
RRID: AB_10913812 (BioLegend Cat. No. 353217) AB_10917385 (BioLegend Cat. No. 353218)
Structure: Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution: T cells, B cells, NK, dendritic cells.
Function: The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor: CCL19 and CCL21.
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Yanagihara S, et al. 1998. J. Immunol. 161:3096. 2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610. 3. Reif K, et al. 2002. Nature 416:94. 4. Nakata B, et al. 2008. Oncology 74:69. 5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed 6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed 7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
Gene ID: 1236
UniProt: View information about CD197 on UniProt.org
Clone: G043H7
Regulatory Status: RUO
Other Names: BLR2, CDw197, EBI1, CMKBR7
Isotype: Mouse IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.