Biolegend, 353311, APC anti-human Perforin Antibody, 25tests

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed Nogimori T, et al. 2023. Front Immunol. 13:1081047. PubMed Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed Alsaleh G, et al. 2020. Elife. 9: . PubMed Guo S, et al. 2021. Stem Cells Int. 2021:9989790. PubMed Han F, et al. 2022. Cent Eur J Immunol. 46:427. PubMed Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed Staats R, et al. 2018. Sleep Breath. 22:211. PubMed
RRID: AB_2571968 (BioLegend Cat. No. 353311) AB_2571969 (BioLegend Cat. No. 353312)
Structure: 70 kD
Distribution: CTL, NK (cytoplasmic granules)
Function: Mediates targeted cell lysis.
Cell Type: T cells, NK cells, Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361. 2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.
Gene ID: 5551
UniProt: View information about Perforin on UniProt.org
Clone: B-D48
Regulatory Status: RUO
Other Names: PRF1, P1, PFP, HPLH2
Isotype: Mouse IgG1