Biolegend, 353802, Purified anti-human CD370 (CLEC9A/DNGR1) Antibody, 100μg

CD370 (CLEC9A), also known as DNGR1, is a type II transmembrane protein consisting of a single extracellular C-type lectin domain (CTLD) that is connected to the transmembrane domain by a stalk region, and an intracellular cytoplasmic tail with immunoreceptor tyrosine-based activation motif (ITAM). In humans, CLEC9A is strictly expressed on BDCA3-positive conventional dendritic cells (cDC) and CD16 - monocytes. CLEC9A recognizes a ubiquitous preformed acid-labile protein associated ligand(s) that is exposed on necrotic cells. CLEC9A ligation is necessary for efficient cross-presentation of dead-cell-associated antigen by recruitment and activation of the tyrosine kinase Syk.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID: AB_10983070 (BioLegend Cat. No. 353802)
Structure: Type II transmembrane glycoprotein; consists of a 35 amino acid (aa) cytoplasmic domain with an ITAM like motif, a 21 aa transmembrane segment, a 185 aa extracellular domain (ECD) that contains a stalk region, and one C-type lectin domain (CTLD).
Distribution: Dendritic subsets including plasmacytoid dendritic cells.
Function: Cross-present antigen to CTL, phagocytosis necrotic cells.
Interaction: Necrotic cells.
Ligand/Receptor: Ubiquitous preformed acid-labile protein associated ligand(s).
Cell Type: Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, MHC Antigens
Antigen References: 1. Poulin LF, et al. 2010. J. Exp. Med. 207:1261. 2. Sancho D, et al. 2009. Nature 458:899. 3. Huysamen C, et al. 2008. J. Biol. Chem. 283:16693.
Gene ID: 283420
UniProt: View information about CD370 on UniProt.org
Clone: 8F9
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: C-type lectin family member 9A, CD370
Isotype: Mouse IgG2a, κ