Biolegend, 354001, Purified anti-human Tim-4 Antibody, 25μg

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th-1 and Th-2) cell-mediated immunity. Tim-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of Tim-1. This binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, Tim-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The Tim-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and is connected to Tim-1-triggered Th2 responsiveness.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TIM-4- IgV Fc, fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 9F4 recognizes the FG loop of human Tim-41.
Application References(PubMed link indicates BioLegend citation): Kobayashi N, et al. 2007. Immunity 27:927.
Product Citations: De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
RRID: AB_11126746 (BioLegend Cat. No. 354002)
Structure: Type 1 transmembrane glycoprotein and mucin domain containing protein 4.
Distribution: Splenic CD14+, macrophages, immature DC.
Function: Tim-1 ligation to Tim-4 induces T cell proliferation, promotes cytokine production, and aids in the regulation of Th2 immunity.
Ligand/Receptor: Phosphatiylserine is the receptor of Tim-4, and Tim-4 is a ligand of Tim-1.
Cell Type: Dendritic cells, Macrophages
Biology Area: Immunology
Antigen References: 1. Kobayashi N, et al. 2007. Immunity 27:927. 2. Meyers JH, et al. 2005. Nat. Immunol. 6:455. 3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454. 4. Shakhov AN, et al. 2004. Eur. J. Immunol. 34:494.
Gene ID: 91937
UniProt: View information about Tim-4 on UniProt.org
Clone: 9F4
Regulatory Status: RUO
Other Names: SMUCKLER (Spleen, mucin-containg, knock-out of lymphotoxin (LT)), TIMD4 (T-cell immunoglobulin and mucin domain containing 4)
Isotype: Mouse IgG1, κ