Biolegend, 354917, Alexa Fluor® 700 anti-human CD21 Antibody, 25tests

CD21 is a 145 kD transmembrane protein also known as complement C3d receptor (C3dR), complement receptor 2 (CR2), and Epstein-Barr virus receptor. CD21 is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells. CD21 is the receptor used by Epstein-Barr virus to infect B cells and is also the complement receptor for C3d. CD21 has also been shown to interact with a number of proteins, including CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1).
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4.
Application References(PubMed link indicates BioLegend citation): Björck P, et al. 1993. Eur. J. Immunol. 23:1771. Frémeaux-Bacchi V, et al. 1996. Eur. J. Immunol. 26:1497. Ling NR, et al. 1995. Clin. Exp. Immunol. 101:369. Wang, C, et al. 2011. BMC Immunol. 12:53. (IHC)
Product Citations: Lu T, et al. 2023. J Med Virol. 95:e28730. PubMed He T, et al. 2022. Liver Int. 42:1287. PubMed Zhao Y, et al. 2022. Front Immunol. 13:1041176. PubMed Guo Q, et al. 2023. Front Public Health. 10:1067342. PubMed Zhang S, et al. 2023. J Clin Med. 12: . PubMed Li H, et al. 2022. Front Immunol. 13:988004. PubMed Li T, et al. 2022. Int J Infect Dis. 122:874. PubMed Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed Song R, et al. 2022. Front Immunol. 13:1028246. PubMed
RRID: AB_2750238 (BioLegend Cat. No. 354917) AB_2750239 (BioLegend Cat. No. 354918)
Structure: Transmembrane protein, multiple SUSHI domains, approximate molecular weight 145 kD
Distribution: Expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, some epithelial cells
Function: Epstein-Barr virus receptor on B cells used for infection
Interaction: CD23, CD19, complement component 3, annexin VI, CD81, iC3b, complement receptor 1, interferon alpha 1
Ligand/Receptor: C3d, EBV
Cell Type: B cells, Dendritic cells, Epithelial cells, T cells, Thymocytes
Biology Area: Cell Biology, Complement, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Kishimoto T, Eds. 1997. Leukocyte Typing VI. Garland Publishing Inc. 2. Moore MD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9194. 3. Szakonyi G, et al. 2001. Science 292:1725. 4. Weis JJ, et al. 1984. Proc. Natl. Acad. Sci. USA 81:881.
Gene ID: 1380
UniProt: View information about CD21 on UniProt.org
Clone: Bu32
Regulatory Status: RUO
Workshop: V CD21.4, VI CD21.5
Other Names: Complement C3d receptor (C3dR), complement receptor 2 (CR2), Epstein-Barr virus receptor
Isotype: Mouse IgG1, κ