Biolegend, 355016, PerCP/Cyanine5.5 anti-human CD124 (IL-4Rα) Antibody, 100tests

CD124, also known as the alpha subunit of IL-4R, is a 140 kD transmembrane glycoprotein. It associates with either the common γ-chain (CD132) to form the type I IL-4R complex, which specifically binds IL-4, or with IL-13Ra1 to form the type II IL-4R heterodimeric complex, which binds and transduces signals from either IL-4 or IL-13.  A truncated form of IL-4Rα exists in the soluble form in biological fluids. CD124 is expressed on human B and T cells as well as a variety of other hematopoietic and non-hematopoietic cells and cell lines.  In B cells, CD124 can bind with IL-4 and IL-13 to regulate IgE antibody production. In T cells, the type I IL-4R (IL-4R/gC) is mostly responsible for Th2 cell expansion by mediating IL-4-dependent activation of the transcription factors in hematopoietic cells. The type II IL-4R (IL-4R/IL-13Ra1) is the main route for non-hematopoietic cell responses to IL-4 or IL-13.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-4Rα Fc chimera
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
RRID: AB_2820001 (BioLegend Cat. No. 355015) AB_2820002 (BioLegend Cat. No. 355016)
Structure: 140 kD type I transmembrane glycoprotein, associates with common γ chain or IL-13 receptor alpha-1 subunit
Distribution: B cells, T cells, endothelial cells, cancer cells
Function: Regulates IgE antibody production in B cells, Th2 cell expansion, and IL-4 or IL-13 induced response in non-hematopoietic cells
Interaction: STAT6
Ligand/Receptor: IL-4, IL-13
Cell Type: B cells, Endothelial cells, T cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: Kashiwada M, et al. 2001. J. Immunol. 167:6382. Gilmour J, et al. 2008. Immunology 124:437. Hage T, et al. 1999. Cell 97:271.
Gene ID: 3566
UniProt: View information about CD124 on UniProt.org
Clone: G077F6
Regulatory Status: RUO
Other Names: IL-4 receptor α subunit
Isotype: Mouse IgG2a, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.