Biolegend, 355102, Purified anti-human CD70 Antibody, 100μg

CD70, also known as CD27L, is a 50 kD type II transmembrane glycoprotein and member of the tumor necrosis factor superfamily. CD70 is expressed on activated T, B and NK cells, activated plasmacytoid dendritic cells (pDCs), and chronic B cell lymphocytic leukemia and large B cell lymphomas. CD70 costimulates T cell proliferation and differentiation. It plays a role in the pDC-induced B cell differentiation. The ligand of CD70 is CD27.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CD70-transfected L cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications for the relevant formats include: blocking of plasmacytoid dendritic cell induced B cell proliferation and Ig secretion1.
Application References(PubMed link indicates BioLegend citation): Shaw J, et al. 2010. Blood 115:3051. (Block)
Product Citations: Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed Zhang X, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1518. PubMed Tian Y, et al. 2019. Cell Rep. 29:4482. PubMed Francescone R, et al. 2020. Cancer Discovery. 11(2):446-479. PubMed Abolhassani H, et al. 2017. J Exp Med . 214(1):91-106. PubMed Ravell JC, et al. 2020. J Clin Invest. 130:507. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID: AB_2561428 (BioLegend Cat. No. 355101) AB_2561429 (BioLegend Cat. No. 355102)
Structure: Type II transmembrane glycoprotein, member of the tumor necrosis factor superfamily, 50 kD
Distribution: Activated T cells and B cells, activated NK cells, activated plasmacytoid dendritic cells, some B cell lymphomas
Function: Role in T cell proliferation, generation of cytolytic and memory T cells, B cell proliferation and differentiation
Ligand/Receptor: CD27
Cell Type: B cells, Dendritic cells, NK cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Bowman MR, et al. 1994. J. Immunol. 152:1756. 2. Shaw J, et al. 2010. Blood 115:3051. 3. Keller AM, et al. 2009. Blood 113:5167.
Gene ID: 970
UniProt: View information about CD70 on UniProt.org
Clone: 113-16
Regulatory Status: RUO
Other Names: CD27 ligand, CD27L, Ki-24 antigen, Tumor necrosis factor ligand superfamily, member 7 (TNFSF7)
Isotype: Mouse IgG1, κ