Biolegend, 357407, APC anti-human CD4 Antibody, 25tests

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CD4 T cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Product Citations: Lafôrets F, et al. 2023. iScience. 26:106514. PubMed Jiang XL, et al. 2021. Nat Commun. 12:897. PubMed Ringelstein-Harlev S, et al. 2023. Eur J Haematol. 110:371. PubMed Sun L, et al. 2023. Mol Med Rep. 27: . PubMed Angiari S, et al. 2020. Cell Metab. 31:391. PubMed Feng R, et al. 2021. Scand J Immunol. 93:e13007. PubMed Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed Zhan Y, et al. 2021. JCI Insight. 6:. PubMed Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed Liu T, et al. 2022. Front Immunol. 13:901349. PubMed Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed Feng Y, et al. 2022. Life (Basel). 12:. PubMed Cheng J, et al. 2021. Biomed Res Int. 2021:6614784. PubMed Lin G, et al. 2021. Mol Med Rep. 23:. PubMed Danzer H, et al. 2020. Elife. 9:00. PubMed Zheng Z, et al. 2021. Biomed Res Int. 2021:5535578. PubMed Yang JY, et al. 2020. J Cell Mol Med. . PubMed Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID: AB_2565659 (BioLegend Cat. No. 357407) AB_2565660 (BioLegend Cat. No. 357408)
Structure: 55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution: Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells
Interaction: Part of the TCR/CD3 complex
Ligand/Receptor: β2 domain from the MHC class II
Cell Type: T cells, Monocytes, Macrophages, NKT cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. 2. Vignali DA. 2010. J. Immunol. 184:5933. 3. Zhou L, et al. 2009. Immunity 30:646. 4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. 5. Zhu J and Paul WE. 2008. Blood 112:1557.
Gene ID: 920
UniProt: View information about CD4 on UniProt.org
Clone: A161A1
Regulatory Status: RUO
Other Names: T4/Leu-3
Isotype: Rat IgG2b, κ
Q: I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
A: TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.