Biolegend, 357504, PE anti-human CD269 (BCMA) Antibody, 100tests

CD269, also known as B cell maturation antigen (BCMA), is a 27 kD, single pass transmembrane protein with one TNFR-Cys repeat on its extracellular domain. CD269 is a B cell maturation factor, essential for the long-term survival of plasma cells. It is expressed by plasmablasts, plasma cells, and germinal center B cells. The ligands of CD269 are BAFF and APRIL, and its cytoplasmic domain binds several of the TRAF family members.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: BCMA-mouse IgG Fc fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Metelo AM, et al. 2022. Cancer Res Commun. 2:158. PubMed Ferguson ID, et al. 2022. Nat Commun. 13:4121. PubMed Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed Chen J, et al. 2020. Genome Biol. 0.980555556. PubMed Zah E, et al. 2020. Nat Commun. 11:2283. PubMed Alturaiki W, et al. 2021. Saudi J Biol Sci. 28:7455. PubMed den Hartog G, et al. 2018. Eur J Immunol. 48:283. PubMed Klein D, et al. 2021. Mol Ther. 29:2053. PubMed Nelke J, et al. 2020. MAbs. 12:1807721. PubMed
RRID: AB_2561925 (BioLegend Cat. No. 357503) AB_2561926 (BioLegend Cat. No. 357504)
Structure: Single pass transmembrane protein, member of the TNFR superfamily, 1 TNFR-Cys domain, 27 kD
Distribution: Plasmablasts, plasma cells, germinal center B cells
Function: B cell maturation factor, essential for survival of plasma cells
Interaction: Associates with several TRAF family members
Ligand/Receptor: BAFF, APRIL
Cell Type: B cells, Plasma cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Coquery CM and Erickson LD. 2012. Crit. Rev. Immunol. 32:287. 2. Notas G, et al. 2012. J. Immunol. 189:4748. 3. Rickert RC, et al. 2011. Immunol. Rev. 244:115. 4. Mesin L, et al. 2011. J. Immunol. 187:2867.
Gene ID: 608
UniProt: View information about CD269 on UniProt.org
Clone: 19F2
Regulatory Status: RUO
Other Names: BCM, TNFRSF17
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.