Biolegend, 358906, PerCP/Cyanine5.5 anti-human CD199 (CCR9) Antibody, 100tests

Human CD199, also known as CCR9, is a member of the G protein coupled receptor family and is involved in T cell development in the thymus and in the gut-associated immune response. It is highly expressed on different stages of thymocytes and upregulated on CD4 + CD8 + cells. Expression of CCR9 on γ/δ T cells in the intraepithelial and small intestine has been reported. The interaction of CCR9 with its ligand CCL25 (TECK, thymus-expressed chemokine) may direct the trafficking of developing T cells in the thymus and the generation of gut-specific immunological memory.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Cells transfected with human CCR9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Liu F, et al. 2016. PLoS Pathog. 12: 1005663. PubMed Hu H, et al. 2014. Proc Natl Acad Sci U S A. 111:13439. PubMed Mohns M, et al. 2015. J Virol. 89: 9748 - 9757. PubMed
RRID: AB_2562448 (BioLegend Cat. No. 358905) AB_2562449 (BioLegend Cat. No. 358906)
Structure: A member of G-protein coupled receptors which contains seven transmembrane domains. Human CCR9 has above 80% amino acid sequence homology with its mouse counterpart.
Distribution: CD4+CD8+ thymocytes, γ/δ T cells, subset of memory T cells
Function: May direct the trafficking of developing T cells in the thymus and generation of gut-specific immunological memory; co-receptor for HIV-1
Ligand/Receptor: CCL25
Cell Type: T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Zaballos A, et al. 1999. J. Immunol. 162:5671. 2. Wurbel MA, et al. 2007. J. Immunol. 178:7598. 3. Wurbel MA, et al. 2006. Eur. J. Immunol. 36:73.
Gene ID: 10803
UniProt: View information about CD199 on UniProt.org
Clone: L053E8
Regulatory Status: RUO
Other Names: GPR 9-6, GPR28, CDw199, Chemokine Receptor 9
Isotype: Mouse IgG2a, κ
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.