Biolegend, 360740, PE anti-human CD16 Recombinant Antibody, 100tests

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).
100tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3683382 (BioLegend Cat. No. 360739) AB_3683382 (BioLegend Cat. No. 360740)
Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils and a subset of T cells
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Interaction: CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC)
Ligand/Receptor: IgG Fc receptor III (FcγRIII)
Cell Type: Macrophages, Monocytes, Neutrophils, NK cells, T cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Rataj F, et al. 2019. Br J Cancer. 120:79-87. Kaufmann M, et al. 2021. Med. 2:296-312. Sigal N, et al. 2022. Methods Mol Biol. 2543:113-128. Cheung P, et al. 2018. Cell. 173:1385. Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. Roussel M, et al. 2021. Cell Reports Medicine. 2:100291. Waugh KA, et al. 2020. Cell Reports. 29:1893-1908.e4.
Gene ID: 22142215
UniProt: View information about CD16 on UniProt.org
Clone: B73.1.Rec
Regulatory Status: RUO
Other Names: FcγRIII, IGFR3, FCG3, FCGR3, FCGRIII, Fc gamma receptor, Fc gamma receptor 3, CD16a
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.