Biolegend, 361102, Purified anti-human/mouse/rat MR1 Antibody, 100μg

MHC-related protein 1, also known as MR1, is a nonclassical MHC class I molecule highly conserved in mammals, particularly in its α1/α2 domains. MR1 is required for the development and expansion of the mucosal-associated invariant T (MAIT) cells. The transcripts for MR1 are ubiquitously expressed, but its expression on the cell surface seems to be ligand-dependent. MR1 is involved in the presentation of vitamin-derived compounds from bacterial biosynthetic pathways.
100μg
Verified Reactivity: Human, Mouse, Rat
Reported Reactivity: Cow
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Soluble complexes of MR1 ectodomain and B2M.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedELISA, IP, Block - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications for the relevant formats include: blocking MR1-dependent activation of MAIT hybridomas, clones, or MAIT TCR-transduced cell lines1-6, detection of folded MR1 conformers3,7, and immunoprecipitation of MR1 from cell lysates7.
Application References(PubMed link indicates BioLegend citation): Young MH, et al. 2013. PLoS One. 8:e53789. (Block) Leeansyah E, et al. 2013. Blood 121:1124. (Block) Kjer-Nielsen L, et al. 2012. Nature 491:717. (Block, ELISA) Reantragoon R, et al. 2012. J. Exp. Med. 209:761. (Block, FC) Gold MC, et al. 2010. PLoS Biol. 8:e1000407. (Block, FC) Huang S, et al. 2009. Proc. Natl. Acad. Sci. USA 106:8290. (Block, FC) Huang S, et al. 2005. J. Biol. Chem. 280:21183. (IP, ELISA, FC)
Product Citations: Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed Wyatt-Johnson SK, et al. 2023. J Neuroinflammation. 20:78. PubMed Vorkas CK, et al. 2022. J Immunol. 208:1042. PubMed Berkson JD, et al. 2020. Immunohorizons. 4:14. PubMed Marquardt I, et al. 2022. Front Microbiol. 12:752549. PubMed Paquin–Proulx D, et al. 2018. PLoS Negl Trop Dis. 12:e0006154. PubMed Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed Dias J, et al. 2018. Proc Natl Acad Sci U S A. 115:E11513. PubMed Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed Flament H, et al. 2021. Nat Immunol. 322:22. PubMed Gherardin NA, et al. 2021. Cancer Immunol Res. 9:612. PubMed Wilgenburg B, et al. 2016. Nat Commun. 7:11653. PubMed Dias J, et al. 2017. Proc Natl Acad Sci U S A. 114:E5434. PubMed Shaler C, et al. 2017. PloS Biol.. 15(6):e2001930. PubMed
RRID: AB_2562969 (BioLegend Cat. No. 361102)
Structure: Member of the MHC class I family, 1 Ig-like C1-type domain, forms heterodimer with B-2 Microglobulin.
Distribution: MR1 transcripts are ubiquitously expressed, but its expression in the cell surface seems to be ligand-dependent.
Function: Antigen presentation, required for development and expansion of the mucosal-associated invariant T (MAIT) cells.
Ligand/Receptor: α-mannosyl ceramide derivatives, 6-formyl pterin, derivatives from bacterial riboflavin biosynthetic pathway).
Biology Area: Immunology, Innate Immunity
Molecular Family: MHC Antigens
Antigen References: 1. Gold MC and Lewinsohn DM. 2013. Nat. Rev. Microbiol. 11:14. 2. Young MH and Gapin L. 2013. Cell Res. 23:460. 3. Gold MC, et al. 2013. Mucosal Immunol. 6:35. 4. Meierovics A, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E3119.
Gene ID: 31401506425119
UniProt: View information about MR1 on UniProt.org
Clone: 26.5
Regulatory Status: RUO
Other Names: Major Histocompatibility Complex Class I-related, HLALS
Isotype: Mouse IgG2a, κ