Biolegend, 362102, Purified anti-human CCX-CKR (CCRL1) Antibody, 100μg

Human CCX-CKR, also known as CCRL1, is a 40 kD, G-protein-coupled, seven transmembrane receptor. CCRL1 is a decoy receptor for CCL2, CCL8, CCL13, CCL19, CCL21 and CCL25 that regulates chemotaxis of lymphocytes and cancer cells. Chemokine binding by CCX-CKR causes its internalization, reducing levels of available chemokine, and thus regulating migration of lymphocytes and cancerous cells. CCX-CKR is expressed by stromal cells of skin-draining lymph nodes, thymic epithelial cells, and immature dendritic cells (DCs). A low CCX-CKR expression in tumor cells correlates with a poor survival rate of breast cancer patients.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length CCX-CKR cDNA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested ICC, IP, Agonist - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications for the relevant formats include: immunoprecipitation of CCX-CKR from cell lysates1, induction of CCX-CKR internalization1 and immunofluorescence1. 13E11 recognizes an epitope located within the first 14 amino acids of CCX-CKR.
Application References(PubMed link indicates BioLegend citation): Takatsuka, S. et al. 2011. J. Pharmacol. Toxicol. Methods. 63:250. (IF, IP, Agonist)
Product Citations: Bryce S, et al. 2016. J Immunol. 196: 3341-3353. PubMed Matti C, et al. 2020. Front Immunol. 11:720. PubMed
RRID: AB_2563201 (BioLegend Cat. No. 362102)
Structure: G-protein-coupled seven transmembrane receptor, 40 kD.
Distribution: Stromal cells of skin-draining lymph nodes, thymic epithelial cells, immature DCs.
Function: C-C type chemokine scavenger and decoy receptor, reduces chemokine availability by sequestering them inside the cell.
Interaction: β-arrestin.
Ligand/Receptor: CCL2, CCL8, CCL13, CCL19, CCL21, and CCL25.
Cell Type: Dendritic cells, Epithelial cells
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Watts AO, et al. 2013. J. Biol. Chem. 288:7169. 2. Comerford I, et al. 2010. Blood. 116:4130.
Gene ID: 51554
UniProt: View information about CCX-CKR on UniProt.org
Clone: 13E11
Regulatory Status: RUO
Other Names: ACKR4, CC-CKR-11, VSHK1, PPR1, CCBP2, CCR11, CKR-11, CCXCKR
Isotype: Mouse IgG2b, κ