Biolegend, 362251, Human CD4 Treg Surface ID Panel, 25tests

This panel allows the identification of regulatory CD4 T cells (Treg) using surface markers, and it is intended to be applied to sort or analyze Treg cells using flow cytometry. The panel does not contain any conjugated PE antibody to leave room to incorporate an antibody of choice in the analysis, using a common and bright fluorophore. Tregs are critical to maintain immune system homeostasis by suppressing the function of other cells. Thus, they help maintain a proper balance between healthy and disease states such as autoimmunity and cancer.
25tests
Verified Reactivity: Human
Formulation: Antibodies supplied in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).7-AAD supplied in PBS, pH 7.2 with 0.09% (w/v) sodium azide.
Preparation: This reagent contains one vial each of 7-AAD, APC/Cyanine7 conjugated anti-human CD3, FITC conjugated anti-human CD4, APC conjugated anti-human CD25, and PE/Cyanine7 conjugated anti-human CD127 (IL-7Ra) at optimal concentration for multicolor flow cytometric analysis. CAUTION: 7-AAD is a potential carcinogen. It is recommended that the user wear protective clothing, gloves, and eye/face protection in order to avoid contact with skin and eyes.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The reagent should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Application Notes: The panel has been verified by NanoString (https://www.nanostring.com) to be compatible with their Vantage 3D™ Immune Cell Profiling Assay for 3D Flow™ Analysis. This product consists of four antibody vials, and one 7-AAD vial. Antibodies include APC/Cyanine7 anti-human CD3, FITC anti-human CD4, APC anti-human CD25, and PE/Cyanine7 anti-human CD127 (IL-7Rα). For a more flexible panel building design, please visit our Muticolor Panel Selector tool. If you prefer another live/dead reagent, please explore our chemical probes and Zombie dyes
RRID: AB_3097045 (BioLegend Cat. No. 362251)
Cell Type: Tregs
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Sudo T, et al. 1993. Proc. Natl. Acad. Sci. USA 90:9125. He YW, Malek TR. 1998. Crit. Rev. Immunol. 18:503. Huster KM, et al. 2004. Proc. Natl. Acad. Sci. USA 101:5610. Taniguchi T, Minami Y. 1993. Cell 73:5. Waldmann T. 1991. J. Biol. Chem. 266:2681. Beverley PC, Callard RE. 1981. Eur. J. Immunol. 11:329. Lanier LL, et al. 1986. J. Immunol. 137:2501-7. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445. Vignali DA. 2010. J. Immunol. 184:5933. Zhou L, et al. 2009. Immunity 30:646. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788. Zhu J, Paul WE. 2008. Blood 112:1557.
Gene ID: 91592035593575
UniProt: View information about CD4 on UniProt.org
Clone: A019D5; BC96; UCHT1; A161A1;
Regulatory Status: RUO
Other Names: Treg Panel
Isotype: Mouse IgG1, κ/Rat IgG2b, κ