Biolegend, 362507, PE anti-human CD56 (NCAM) Antibody, 25tests

CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human myotube cells
Formulation: µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.4 µg per million cells in 100 µl volume. For flow cytometric staining using test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Walsh FS, et al. 1981. Nature 289:60. (FC) Pavlath GK, et al. 1986. J. Cell Biol. 102:124. (FC) Pavlath GK, et al. 1989. Nature 337:570. (FC) Pulido R, et al. 1988. J. Immunol. 140:3851. (FC)
Product Citations: Wu X, et al. 2023. iScience. 26:106559. PubMed Zheng G, et al. 2021. Signal Transduct Target Ther. 6:236. PubMed Kim MY, et al. 2021. JCI Insight. 6:. PubMed Pan R, et al. 2022. Cell. 185:1521. PubMed Segal JM, et al. 2019. Nat Commun. 10:3350. PubMed Ding L, et al. 2021. Cancers (Basel). 13:. PubMed Bayley JP, et al. 2022. PLoS One. 17:e0274478. PubMed Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed Brighton PJ et al. 2017. eLife. 6 pii: e31274. PubMed Wu X, et al. 2020. Cancers (Basel). 12:. PubMed Scherer SD, et al. 2021. Breast Cancer Res. 23:100. PubMed Chen P, et al. 2021. Front Immunol. 12:689019. PubMed Chakraborty S, et al. 2022. Sci Transl Med. . PubMed Leibel SL, et al. 2022. iScience. 25:103797. PubMed Wu X, et al. 2021. Front Immunol. 12:731767. PubMed Liu J, et al. 2022. iScience. 25:105509. PubMed Vanoni G, et al. 2022. Bio Protoc. 218:. PubMed Dhar P, et al. 2021. Commun Biol. 4:905. PubMed Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed Shaffer TM, et al. 2020. J Nucl Med. 1348:61. PubMed Liu B, et al. 2019. Mol Med Rep. 20:2823. PubMed Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed Wang F, et al. 2021. Genomics Proteomics Bioinformatics. 19:208. PubMed Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
RRID: AB_2563924 (BioLegend Cat. No. 362507) AB_2564161 (BioLegend Cat. No. 362524) AB_2563925 (BioLegend Cat. No. 362508)
Structure: Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution: NK cells, T subset, neural tissue, some LGL and myeloid leukemias
Function: Adhesion
Ligand/Receptor: Heparan sulfate
Antigen References: 1. Lanier L, et al. 1991. J. Immunol. 146:4421 2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71 3. Cremer H, et al. 1994. Nature 367:455.
Gene ID: 4684
UniProt: View information about CD56 on UniProt.org
Clone: 5.1H11
Regulatory Status: RUO
Other Names: Leu-19, NKH1, NCAM-1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.