Biolegend, 365503, PE anti-human FCGR2A/CD32A Antibody, 25tests

CD32A (FCGR2A) is a 40 kD, type I protein with 2 C2-set domains the extracellular region and 1 ITAM in the intracellular region. CD32A binds monomeric IgG with low affinity but is very efficient binding immune complexes.  FCGR2A is expressed by myeloid cells such as macrophages, monocytes, neutrophils, eosinophils and dendritic cells; CD32a is also expressed by epithelial cells, platelets and activated CD4+ T cells. CD32a is involved in phagocytosis and clearing of immune complexes.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: FCGR2A-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This clone does not recognizes CD32B-transfected cell
RRID: AB_2904394 (BioLegend Cat. No. 365503) AB_2904394 (BioLegend Cat. No. 365504)
Structure: Member of the Ig superfamily, 40 kD, type I protein, 2 C2-set domains, 1 ITAM
Distribution: Macrophages, monocytes, neutrophils, eosinophil, platelets, and CD4+ T Cells
Function: Phagocytosis, cytotoxicity, platelet aggregation
Ligand/Receptor: Aggregated IgG, immune complexes
Cell Type: Eosinophils, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets
Biology Area: Adaptive Immunity, Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: Hubbard J. et al. 2020. J Exp Med. 217:e20200359 Newling M, et al. 2020. Clin Exp Immunol. 199:39-49 Maria H, et al. 2018. Front Immunol. 9:2814 Descours B, et al. 2017. Nature. 543:564-567
Gene ID: NA
UniProt: View information about FCGR2A/CD32A on UniProt.org
Clone: S20004A
Regulatory Status: RUO
Other Names: FcγRIIa, FCGR2A, CD32A
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.