Biolegend, 366918, Biotin anti-human IgG Fc Recombinant Antibody, 100μg

IgG Fc is a homodimer composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Anti-human IgG Fc antibody (clone QA19A42) may cross-react with bovine IgG. Anti-human IgG Fc antibody (clone M1310G05) does not exhibit this cross-reactivity.
Product Citations: Jaycox JR, et al. 2023. Nat Commun. 14:1299. PubMed
RRID: AB_2890797 (BioLegend Cat. No. 366918)
Structure: Homodimer formed by the constant region of the IgG heavy chain
Ligand/Receptor: CD16, CD32, CD64
Cell Type: B cells, Basophils, Dendritic cells, Macrophages, Mast cells, Neutrophils, NK cells, Platelets
Biology Area: Adaptive Immunity, Cell Biology, Immunology, Innate Immunity
Molecular Family: Fc Receptors
Antigen References: Paul WE. 2003. Fundamental Immunology. Lippincott Williams & Wilkins. Philadelphia PA.
Gene ID: 3500
UniProt: View information about IgG Fc on UniProt.org
Clone: QA19A42
Regulatory Status: RUO
Other Names: Immunoglobulin G
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.