Biolegend, 368502, Purified anti-human CD45 Antibody, 100μg

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBMC
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Application References(PubMed link indicates BioLegend citation): Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33. Csiba A, et al. 1984. Br. J. Cancer 50:699. Tchilian EZ, et al. 2001. J. Immunol. 166:1308. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations: Valet C, et al. 2022. J Clin Invest. 132: . PubMed Korn MA, et al. 2020. J Immunol. 205:2595. PubMed Wang J, et al. 2020. Sci Adv. 6:eaax3223. PubMed Mann ER, et al. 2020. Sci Immunol. :5. PubMed Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed Zhao L, et al. 2018. Nat Med. 24:1536. PubMed Walker DG, et al. 2019. Cells. 8. PubMed Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed Xie S, et al. 2020. Ann Transl Med. 1.384027778. PubMed Balakrishnan A, et al. 2019. Sci Rep. 9:7933. PubMed Donnelly C, et al. 2018. Sci Rep. 0.625. PubMed Wäldchen F, et al. 2020. Nat Commun. 1.074305556. PubMed Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
RRID: AB_2566240 (BioLegend Cat. No. 368502)
Structure: Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
Distribution: Hematopoietic cells, except circulating erythrocytes or platelets.
Function: Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Ligand/Receptor: Galectin-1, CD2, CD3, and CD4.
Cell Type: B cells, Dendritic cells, Neutrophils
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788
UniProt: View information about CD45 on UniProt.org
Clone: 2D1
Regulatory Status: RUO
Other Names: Leukocyte Common Antigen (LCA), T200
Isotype: Mouse IgG1, κ