Biolegend, 368507, FITC anti-human CD45 Antibody, 25tests

CD45 is a 180 - 240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes or platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human PBMC
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application Notes: It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.
Application References(PubMed link indicates BioLegend citation): Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33. Csiba A, et al. 1984. Br. J. Cancer 50:699. Tchilian EZ, et al. 2001. J. Immunol. 166:1308. Lee MS, et al. 2004. Int. Immunol. 16:1109.
Product Citations: Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed Wu X, et al. 2023. iScience. 26:106559. PubMed Aabling RR, et al. 2023. Regen Ther. 23:67. PubMed Iwahashi N, et al. 2022. J Clin Endocrinol Metab. 107:2439. PubMed Zhang T, et al. 2022. IUBMB Life. 74:1081. PubMed Hardman CS, et al. 2022. Nat Commun. 13:7535. PubMed Liu X, et al. 2020. Exp Ther Med. 1.258333333. PubMed Radhakrishnan S, et al. 2019. Mol Med Rep. 20:813. PubMed Tu S, et al. 2020. Cellular Signalling. 73:109695. PubMed Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed Wu S, et al. 2020. Stem Cells Int. 2019:1515040. PubMed Sun W, et al. 2019. Exp Ther Med. 17:2199. PubMed Hijmans JG, et al. 2019. J Am Heart Assoc. 8:e011134. PubMed Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed Bhattacharya M, et al. 2020. Infect Immun. :88. PubMed Shen C, et al. 2021. Front Immunol. 12:680055. PubMed Xu P, et al. 2020. Cancer Immunol Res. 8:1193. PubMed Escudero–Pérez B, et al. 2019. JCI Insight. 4:e126070. PubMed Zhan L, et al. 2022. Cancer Lett. 529:37. PubMed Meng X, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed Motedayyen H, et al. 2017. BMC Res Notes. . 10.1186/s13104-017-2880-6. PubMed He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed Felker S, et al. 2022. JCI Insight. 7:. PubMed Gleitz HFE, et al. 2021. STAR Protocols. 2(2):100538. PubMed Lin T, et al. 2021. PLoS Pathog. 17:e1009557. PubMed Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed Matsuo S, et al. 2021. PLoS One. e0247595:16. PubMed Togami K, et al. 2019. J Clin Invest. 129:5005. PubMed Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
RRID: AB_2566367 (BioLegend Cat. No. 368507) AB_2566368 (BioLegend Cat. No. 368508)
Structure: Type I transmembrane protein, 180 - 240 kD (multiple isoforms)
Distribution: Hematopoietic cells, except circulating erythrocytes or platelets.
Function: Tyrosine phosphatases, signaling, co-stimulation (co-inhibition), TCR and BCR mediated activation.
Ligand/Receptor: Galectin-1, CD2, CD3, and CD4.
Cell Type: B cells, Dendritic cells, Neutrophils
Biology Area: Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Protein Kinases/Phosphatase, TCRs
Antigen References: 1. Thomas M. 1989. Annu. Rev. Immunol. 7:339. 2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
Gene ID: 5788
UniProt: View information about CD45 on UniProt.org
Clone: 2D1
Regulatory Status: RUO
Other Names: Leukocyte Common Antigen (LCA), T200
Isotype: Mouse IgG1, κ