Biolegend, 368613, PE/Cyanine7 anti-human KLRG1 (MAFA) Antibody, 25tests

Killer cell lectin-like receptor subfamily G member (KLRG1) is a 30 kD, type II membrane glycoprotein with one C-type lectin domain and one immunoreceptor tyrosine-based inhibitory motif (ITIM). KLRG1 is expressed by subsets of natural killer cells (NKs) and effector and memory T cells. It inhibits cell activation and proliferation and is also a marker of T cell senescence. The binding of KLRG1 to E-, N-, or R- cadherins blocks phosphorylation of AKT and increases the expression of cell cycle inhibitors.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human KLRG1-transfected cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations: Dege C, et al. 2020. Developmental Cell. 53(2):229-239. PubMed Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed O'Connor MH, et al. 2021. Commun Biol. 4:563. PubMed
RRID: AB_2728370 (BioLegend Cat. No. 368613) AB_2728371 (BioLegend Cat. No. 368614)
Structure: Type II membrane glycoprotein, one C-type lectin domain, one ITIM, 30 kD.
Distribution: Subsets of natural killer cells, effector and memory T cells.
Function: Inhibits cell activation and proliferation
Interaction: AKT.
Ligand/Receptor: E-, N-, R- cadherins.
Cell Type: NK cells, T cells
Biology Area: Immunology
Antigen References: 1. Shi L, et al. 2014. J. Immunol. 192:649.
Gene ID: 10219
UniProt: View information about KLRG1 on UniProt.org
Clone: 14C2A07
Regulatory Status: RUO
Other Names: CLEC15A, MAFA-L, MAFA-2F1, MAFA-LIKE, 2F1
Isotype: Mouse IgG2a, κ