Biolegend, 369501, Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody, 25μg

ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:500-1: 5000 diluted (0.1-1.0 µg/ml). For flow cytometric staining, the suggested use of this reagent is ≤ 5.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone 6B8B69 was found to strongly cross-react with mouse ERK1/2 Phospho (Thr202/Tyr204) when tested in-house on C57BL/6 mouse splenocytes.
Product Citations: Recabal A, et al. 2020. Journal of Neurochemistry. 156(2):182-199. PubMed
RRID: AB_2721734 (BioLegend Cat. No. 369501) AB_2721735 (BioLegend Cat. No. 369502)
Structure: ERK1 and ERK2 encode 42 and 44 kD protein kinases and share 85% homology.
Distribution: Cytoplasm and the nucleus.
Function: ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1 and ERK2 are phosphorylated at Thr202 and Tyr204; and in turn transduce signals to downstream substrates.
Interaction: ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: 1. Futran AS, et al. 2013. Curr. Biol. 23:R972. 2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320. 3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299. 4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320. 5. Torii S, et al. 2004. J. Biochem. 136:557. 6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814.
Gene ID: 5594
UniProt: View information about ERK1/2 on UniProt.org
Clone: 6B8B69
Regulatory Status: RUO
Other Names: P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
Isotype: Mouse IgG2a, κ