Biolegend, 371202, Purified anti-human CD357 (GITR) Antibody, 100μg

GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18). GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR binds GITRL and is involved in the development of regulatory T cells and to regulate the activity of Th1 subsets.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human GITR-Fc chimera
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Tavazoie MF, et al. 2018. Cell. 172:825. PubMed Thrash EM, et al. 2020. STAR Protoc. 1:100055. PubMed
RRID: AB_2572031 (BioLegend Cat. No. 371202)
Structure: TNFR superfamily, 234-amino acid type I transmembrane protein, 25 kD.
Distribution: Activated lymphocytes.
Function: Interacts with TRAF's 1-3, but not TRAF 5 or 6, mediates NF-κB activation through the TRAF2/NIK pathway.
Ligand/Receptor: GITRL
Cell Type: Lymphocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. van der Werf N, et al. 2011. J. Immunol. 187:1411. 2. Shimizu J, et al. 2002. Nat. Immunol. 3:135. 3. McHugh RS, et al. 2002. Immunity 16:311. 4. Kwon B, et al. 1999. J. Biol. Chem. 274:6056.
Gene ID: 8784
UniProt: View information about CD357 on UniProt.org
Clone: 108-17
Regulatory Status: RUO
Other Names: TNFRSF18, Activation-inducible TNFR, AITR, Glucocorticoid-inducible TNFR
Isotype: Mouse IgG2a, κ