Biolegend, 372206, FITC anti-human/mouse Granzyme B Recombinant Antibody, 100tests

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed Liu N, et al. 2023. Cell Death Dis. 14:170. PubMed Li G, et al. 2023. Cell Signal. 109:110729. PubMed Woan KV, et al. 2021. Cell Stem Cell. 28:2062. PubMed Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed Dong L, et al. 2021. Cancer Cell. . PubMed Lamichhane R, et al. 2020. Eur J Immunol. 50:178. PubMed Zhao B, et al. 2020. Nat Commun. 11:908. PubMed Klarquist J, et al. 2021. Cell Rep. 36:109591. PubMed Li K, et al. 2022. J Immunother Cancer. 10:. PubMed Liu H, et al. 2020. Cancer Cell. 37(3):324-339. PubMed Jiang H, et al. 2021. Oncoimmunology. 10:1943180. PubMed Yang P, et al. 2022. Nat Commun. 13:5782. PubMed Li H, et al. 2021. Adv Sci (Weinh). 2001596:8. PubMed Sun Y, et al. 2020. Cell. 184(2):404-421.e16. PubMed Takahashi F, et al. 2022. iScience. 25:104278. PubMed Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed Wu J, et al. 2021. STAR Protoc. 2:101022. PubMed Li F, et al. 2022. Nat Commun. 13:4334. PubMed Su Y, et al. 2020. Cell. 1479:183. PubMed Lu H, et al. 2020. Am J Reprod Immunol. 83:. PubMed Shen H, et al. 2022. Nat Commun. 13:5013. PubMed Li M, et al. 2020. Virol Sin. 35:588. PubMed Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed Ye Y, et al. 2020. Genome Med. 0.557638889. PubMed Lamichhane R et al. 2019. Cell Rep. 28(12):3061-3076 . PubMed
RRID: AB_2687029 (BioLegend Cat. No. 372205) AB_2687030 (BioLegend Cat. No. 372206)
Structure: 32 kD serine protease
Distribution: Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells
Function: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction: Caspase-3
Ligand/Receptor: Mannose-6-phosphate receptor
Cell Type: Neutrophils, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: 1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. 2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. 3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. 4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID: 300214939
UniProt: View information about Granzyme B on UniProt.org
Clone: QA16A02
Regulatory Status: RUO
Other Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype: Mouse IgG1, κ