Biolegend, 372219, Alexa Fluor® 647 anti-human/mouse Granzyme B Recombinant Antibody, 25tests

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
25tests
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Product Citations: Mondal N, et al. 2019. J Biol Chem. 18465:294. PubMed Scopa C, et al. 2023. Nat Commun. 8021:14. PubMed
RRID: AB_2728386 (BioLegend Cat. No. 372219) AB_2728387 (BioLegend Cat. No. 372220)
Structure: 32 kD serine protease
Distribution: Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells
Function: Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction: Caspase-3
Ligand/Receptor: Mannose-6-phosphate receptor
Cell Type: T cells, NK cells, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Proteases, Enzymes and Regulators
Antigen References: 1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203. 2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885. 3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610. 4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.
Gene ID: 300214939
UniProt: View information about Granzyme B on UniProt.org
Clone: QA16A02
Regulatory Status: RUO
Other Names: Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype: Mouse IgG1, κ