Biolegend, 373307, Alexa Fluor® 647 anti-human HLA-C Antibody, 25tests

HLA-C belongs to the HLA class I heavy chain paralogues. MHC class I heavy chains associate with β2-microglobulin and are expressed on nearly all nucleated cells. MHC class I molecules are involved in the presentation of antigens to CD8+ T cells and thereby play an important role in cell-mediated immune responses and immune surveillance. Detailed characterization of the DT-9 clone indicates that it cross-reacts with HLA-E and certain HLA-A and HLA-B allotypes.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified tamarin MHC class I molecules, corresponding to human HLA-C
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Corrah et al. 2011. J. Virol. 85: 3367. Apps et al. 2013. Science. 340:87. Braud et al. 1998. Curr. Biol. 8:1. Thomas R, et al. 2009. Nat. Genet. 41:1290. (HLA-A/B allotype cross-reactivity)
Product Citations: Wang R, et al. 2023. Emerg Microbes Infect. 2185467:12. PubMed
RRID: AB_2894582 (BioLegend Cat. No. 373307) AB_2894582 (BioLegend Cat. No. 373308)
Structure: Ig superfamily
Distribution: Nearly all cells
Function: Antigen peptide presentation
Ligand/Receptor: TCR, CD8
Biology Area: Immunology
Molecular Family: MHC Antigens
Gene ID: 3107
UniProt: View information about HLA-C on UniProt.org
Clone: DT-9
Regulatory Status: RUO
Other Names: HLAC, MHC Class I Antigen C
Isotype: Mouse IgG2b, κ