Biolegend, 375140, Brilliant Violet 421™ anti-human CD159a (NKG2A) Antibody, 100tests

CD159a, also known as NKG2A or KLRC1, is a type II transmembrane protein. It belongs to the killer cell lectin-like receptor family also known as the NKG2 family. It is expressed on NK and NKT cells and T cell subsets. NKG2A is an inhibitory NK cell receptor and it is expressed as a heterodimer with CD94. In human, NKG2A/CD94 complex interacts HLA-E on target cells and inhibits T cells and NK cell effector functions. Recent studies showed that NKG2A blockade enhances the anti-tumor effect of NK cell and CD8 + T cells.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human NKG2A/CD94-transfectants
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: Clone S19004C does not cross-react with mouse CD159a.
RRID: AB_2941547 (BioLegend Cat. No. 375139) AB_2941547 (BioLegend Cat. No. 375140)
Structure: Type II transmembrane proteins of the C-type lectin superfamily
Distribution: NK cells, NKT cells, T cell subsets
Function: Inhibiting NK cell receptor
Ligand/Receptor: HLA-E
Cell Type: Lymphocytes, NK cells, NKT cells, T cells
Biology Area: Immuno-Oncology, Immunology
Molecular Family: Immune Checkpoint Receptors
Antigen References: Brooks AG, et al. 1999. J Immunol. 162:305-13. Kaiser BK, et al. 2008. Proc Natl Acad Sci U S A. 105: 6696-701. Braud VM, et al. 2003. Trends Immunol. 24:162-4. Kamiya T, et al. 2019. J Clin Invest. 129:2094. André P, et al. 2018. Cell. 175:1731.
Gene ID: 3821
UniProt: View information about CD159a on UniProt.org
Clone: S19004C
Regulatory Status: RUO
Other Names: Killer Cell Lectin Like Receptor C1, KLRC1, NK Cell Receptor A
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.