Biolegend, 376002, Purified anti-human CD59 Recombinant Antibody, 100μg

CD59 is a 19-25 kD glycosylphosphatidylinositol (GPI)-anchored glycoprotein also known as protectin, MACIF, and H19. It is broadly expressed on hematopoietic and non-hematopoietic cells. CD59 inhibits the cytolytic activity of complement by binding to C9, inhibiting incorporation into C5b-8, and preventing the terminal steps in complement polymerization of the membrane attack complex. CD59 has also been reported to play a role in T cell activation.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2890825 (BioLegend Cat. No. 376002)
Structure: Ly6 superfamily, single chain GPI-linked membrane protein, 19-25 kD
Distribution: All cell types
Function: Blocks complement-mediated lysis by inhibiting MAC formation
Ligand/Receptor: Complement components C8, C9
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Huang Y, et al. 2006. J. Biol. Chem. 281(37):27398-27404.
Gene ID: 966
UniProt: View information about CD59 on UniProt.org
Clone: QA19A13
Regulatory Status: RUO
Other Names: Protectin, H19, 1F-5Ag, HRF20, MACIF, MIRL, P-18
Isotype: Mouse IgG2a, κ