Biolegend, 376502, Purified anti-human CD144 Recombinant Antibody, 100μg

CD144, also known as VE-cadherin and cadherin-5, is a 140 kD glycoprotein which is composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region. It is a calcium-dependent transmembrane cell-cell adhesion molecule localized at the intercellular boundaries of endothelial cells, hematopoietic stem cells, and perineurial cells. It functions as a classic cadherin by mediating homophilic adhesion and functions as a plasma membrane attachment site for the cytoskeleton. CD144 is thought to play a role in vascular development, permeability, and remodeling.
100μg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines.
RRID: AB_2904407 (BioLegend Cat. No. 376502)
Structure: Member of the cadherin family; calcium-dependent transmembrane cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
Distribution: Endothelial cells, hematopoietic stem cells, perineurial cells
Function: Mediates calcium-dependent homophilic cell adhesion, plays fundamental roles in microvascular permeability and in the morphogenic and proliferative events associated with angiogenesis
Cell Type: Endothelial cells
Biology Area: Cell Adhesion, Cell Biology, Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Taddei A, et al. 2008. Nat. Cell Biol. 10:923. 2. Gavard J, et al. 2006. Nat. Cell Biol. 8:1223. 3. Kim I, et al. 2005. Blood 106:903. 4. Suzuki S, et al. 1991. Cell Regul. 2:261.
Gene ID: 1003
UniProt: View information about CD144 on UniProt.org
Clone: QA20A44
Regulatory Status: RUO
Other Names: VE-Cadherin, cadherin-5, CDH5
Isotype: Mouse IgG1, κ