Biolegend, 376911, Ultra-LEAF™ Purified anti-human TLR7 Antibody, 100μg

Toll-like receptor (TLR) 7, also known as CD287 is a type 1 transmembrane protein, member of the TLR receptor family. TLR7 is expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes and plays an important role in the innate immune response by binding single-stranded RNA (ssRNA). TLR7 signals through MyD88, which leads to the activation and secretion of IFN-α/β and other inflammatory cytokines.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by blocking. > 80% blocking was observed with 1 µg antibody per well. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ug per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone S18024F can be used for blocking R848-activated IL8 cytokine secretion (tested on 293XL/TLR7 cell line) and for intracellular flow cytometry.
RRID: AB_2936679 (BioLegend Cat. No. 376911) AB_2936679 (BioLegend Cat. No. 376912)
Structure: Type 1 transmembrane protein, member of the Toll-like receptor (TLR) family
Distribution: Expressed intracellularly in plasmacytoid dendritic cells, myeloid cells, and B lymphocytes, Monocytes
Function: Pattern recognition receptor that detects single-stranded RNA, induces proinflammatory cytokine production
Interaction: B cells, Dendritic cells
Ligand/Receptor: Single-stranded RNA
Cell Type: B cells, Dendritic cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Innate Immune Signaling
Gene ID: 51284
UniProt: View information about TLR7 on UniProt.org
Clone: S18024F
Regulatory Status: RUO
Other Names: Toll-like receptor (TLR) 7, CD287
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.