Biolegend, 377110, PE anti-human HVCN1 Antibody, 100tests

Hydrogen voltage-gated channel 1 (HVCN1) is a voltage gated, proton-selective channel expressed in immune cells, spermatozoa, and upper respiratory tract epithelial cells. HVCN1 mediates proton conductance to prevent membrane depolarization and cytoplasmic acidification. In phagocytes, it is required for the oxidative burst that underlies microbial killing, and in B cells, it facilitates antibody production. HVCN1 can also regulate functions in other cells including spermatozoa.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human HVCN1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Structure: Transmembrane protein with four helix transmembrane domain and a coiled coil c-terminal cytoplasmic domain
Distribution: Immune cells (B-cells, Neutrophils, Monocytes), spermatozoa, upper respiratory epithelium
Function: Proton transporter
Molecular Family: Ion Channels
Antigen References: Zhu X, et al. 2013. BMC Immunol. 14:24. Hondares E, et al. 2014. Proc. Natl. Acad. Sci. 111:50. Okochi Y, et al. 2021. Int. J. Mol. Sci. 22:2620. Wang Y, et al. 2011. Biochem. Biophys. Res. Commun. 2: 412. Cherny VV, et al. 2015. J. Gen. Physiol. 5: 146. De Coursey TE. 2015. Biochemistry. 54: 21.
Gene ID: 84329
UniProt: View information about HVCN1 on UniProt.org
Clone: W23068A
Regulatory Status: RUO
Other Names: Hydrogen voltage gated channel 1, HV1, VSOP, voltage sensor domain-only protein
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.