Biolegend, 377818, PerCP/Fire™ 780 anti-human CD28 Antibody, 100tests

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CD28 transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Fire™ 780 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Fire™ 780 has a maximum excitation of 478 nm and a maximum emission of 780 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone S20013B antibody can completely block the binding of clone CD28.2 on target. Clone S20013B can partially block Clone S20013F on target. Clone S20013B can increase anti-CD3 induced human peripheral T-cell proliferation. Clone S20013B is a superagonistic antibody raised towards human CD28, and can induce activation, proliferation of human peripheral T cells without anti-CD3 stimulation.
RRID: AB_3683416 (BioLegend Cat. No. 377817) AB_3683416 (BioLegend Cat. No. 377818)
Structure: Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Distribution: Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
Function: T cell costimulation
Ligand/Receptor: CD80, CD86
Cell Type: Lymphocytes, T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area: Adaptive Immunity, Cell Proliferation and Viability, Costimulatory Molecules, Immuno-Oncology, Immunology
Molecular Family: CD Molecules
Antigen References: Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York. June CH, et al. 1994. Immunol Today. 15:321-31. Linsley PS and Ledbetter JA. 1993. Annu Rev Immunol. 11:191-212.
Gene ID: 940
UniProt: View information about CD28 on UniProt.org
Clone: S20013B
Regulatory Status: RUO
Other Names: T44, Tp44
Isotype: Mouse IgG1, κ