Biolegend, 378119, PE anti-human VISTA (PD-1H) Antibody, 25tests

VISTA (V-domain Ig suppressor of T cell activation), also known as PD-1H, is a type I transmembrane protein with a single extracellular IgV domain. VISTA is expressed by a subset of T cells, macrophages, dendritic cells, neutrophils, and NK cells. It was reported that in certain tumor environment, there is low-pH dependent interaction between VISTA and PSGL1. However, the physiological binding partner for VISTA and the mechanism of its action are not clearly understood. VISTA is considered as a checkpoint regulator and a promising target for cancer immunotherapy.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human VISTA protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool).
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3720548 (BioLegend Cat. No. 378119) AB_3720548 (BioLegend Cat. No. 378120)
Structure: Ig V-domain like
Distribution: Hematopoietic cells, monocytes, macrophages, dendritic cell, granulocytes, subsets of T cells
Function: Suppressing T cell activation
Cell Type: Dendritic cells, Granulocytes, Lymphocytes, Macrophages, Monocytes, T cells
Biology Area: Immunology, Inhibitory Molecules
Antigen References: Wang Li, et al. 2011. J Exp Med. 208:577-92. Nowak EC, et al. 2017. Immunol Rev. 276:66-79. Flies DB, et al. 2014. J Clin Invest. 124:1966-75.
Gene ID: 64115
UniProt: View information about VISTA on UniProt.org
Clone: A17002B
Regulatory Status: RUO
Other Names: V-Set Immunoregulatory Receptor (VSIR), B7H5, GI24, B7-H5, Dies1, PD-1H, SISP1, VISTA, PP2135, C10orf54, DD1alpha
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.