Biolegend, 378402, Purified anti-human TMPRSS2 Antibody, 100μg

TMPRSS2 is a membrane-anchored protein that belongs to the serine protease family and is involved in many physiological and pathological processes. TMPRSS2 participates in proteolytic cascades and is important in normal physiological function of the prostate. Autocleavage leads to secretion of the protease domain. TMPRSS2 is involved in extracellular matrix disruption and metastasis of prostate cancer cells and activates several substrates, including pro-hepatocyte growth factor/HGF, the protease activated receptor-2/F2RL1 or matriptase/ST14. TMPRSS2 promotes uptake of SARS-CoV-1, MERS-CoV, and SARS-CoV-2 viral particles of by proteolytic cleavage of ACE2 receptor and cleavage of coronavirus spike glycoproteins.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: huTMPRSS2 transfectant
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IHC-P- Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2910437 (BioLegend Cat. No. 378402)
Structure: Single-pass type II membrane protein
Distribution: Cell membrane, activated by cleavage and secreted
Function: Hydrolase, protease, serine protease
Interaction: ACE2, HGF, F2RL1, ST14
Cell Type: Epithelial cells, Platelets
Biology Area: Cancer Biomarkers, Cell Biology, COVID-19
Molecular Family: Proteases
Antigen References: Hoffman M, et al. 2020. Cell. 181:271-280. Yu J, et al. 2010. Cancer Cell. 17:443-54. Shen LW, et al. 2017. Biochimie. 142:1-10.
Gene ID: 7113
UniProt: View information about TMPRSS2 on UniProt.org
Clone: S20014A
Regulatory Status: RUO
Other Names: Transmembrane protease serine 2
Isotype: Mouse IgG1, κ