Biolegend, 379407, Brilliant Violet 421™ anti-human IL-1RL1 (ST2) Antibody, 25tests

IL1RL1 is a member of the Toll-like receptor superfamily the receptor for Interleukin-33. It is a 556 amino acid single-pass type I membrane protein. ST2 exists in two forms as splice variants: a soluble form, which acts as a decoy receptor and sequesters free IL-33, and a membrane-bound form (ST2), which activates the MyD88/NF-κB signaling pathway. Signaling through IL1RL1 drives cytokine production in mast cells, basophils, eosinophils, invariant NKT and NK cells, Th2 lymphocytes, and type 2 innate immune cells. IL-33 signaling is required for maintaining the suppressive function of regulatory T cells.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human IL-1RL1, AA19-328
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
RRID: AB_3716918 (BioLegend Cat. No. 379407) AB_3716918 (BioLegend Cat. No. 379408)
Structure: Single-pass type I membrane protein
Distribution: Th2 lymphocytes, mast cells, eosinophils, basophils, smooth muscle cells, endothelial cells
Function: Cytokine signaling
Interaction: Signaling requires association of the coreceptor IL1RAP. Recruits MYD88, IRAK1, IRAK4, and TRAF6.
Ligand/Receptor: IL-33
Cell Type: Basophils, Endothelial cells, Eosinophils, Lymphocytes, NK cells, Th2, Tregs
Biology Area: Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: Schmitz J, et al. 2005. Immunity. 23: 479-490. Brint E, et al. 2004. Nat Immun. 5:373-379. Griesenauer B, et al. 2017. Front Immunol. 8:475.
Gene ID: 9173
UniProt: View information about IL-1RL1 on UniProt.org
Clone: A16008J
Regulatory Status: RUO
Other Names: IL-33R, ST2, IL-1RL1
Isotype: Mouse IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.