Biolegend, 379908, PE/Cyanine7 anti-human CD11b Recombinant Antibody, 100tests

CD11b (integrin alpha M subunit) is a type I transmembrane glycoprotein. CD11b combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/beta 2, also known as Mac-1 and complement receptor type 3 (CR3). Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen. CD11b is primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells.
100tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: The binding of clone QA20A58 can be completely blocked by reference clone. Other anti-hCD11b clone ICRF44 does not affect the binding of clone QA20A58 to target.
RRID: AB_2941554 (BioLegend Cat. No. 379907) AB_2941554 (BioLegend Cat. No. 379908)
Structure: Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells
Function: Adhesion, chemotaxis, phagocytosis, neutrophil activation
Ligand/Receptor: ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type: B cells, Dendritic cells, Granulocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Innate Immunity
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. Springer TA. 1994. Cell. 76:301-14. Coxon A, et al. 1996. Immunity. 5:653-66.
Gene ID: 3684
UniProt: View information about CD11b on UniProt.org
Clone: QA20A58
Regulatory Status: RUO
Other Names: alpha-chain of C3bi receptor, alphaM, C3bi receptor, CD11b, gp155/95, integrin a, M-chain, Mac-1
Isotype: Mouse IgG2a, κ